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An Open Source command-line OPL3 MIDI player application for Linux and Windows OSes
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ProteinMusic is a Java program converting DNA sequences into music.
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ttrk (tektracker) is a console MIDI sequencer with a tracker-style step editor.
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Playmidi is a curses and X11-based MIDI file player for Linux.
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xmms-timidity is a MIDI plugin for XMMS.
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TiMidity++ is a software synthesizer.